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Across 1. Pinpoint, small, moderate or large. 2. Anionic dye: india ink, nigrosin, eosin Y and Congo red 4. Selective ingredient in MSA plate: 7.5% 8. Reduction of sodium thiosulfate and putrefaction of cysteine yields this end product of interest. 11. KIA/: red/black/lift & cracks 12. Polar tuft of flagella. 13. EPI of Voges-Prokauer test. 16. KIA/: yellow/black/lift & cracks 18. Hydrolytic enzyme that produces golden brown clearing on SA. 21. Non-spore forming, glucose-fermenting Gram-negative bacilli. 22. Substrate hydrolyzed in Milk Agar. 23. TSA, BHIB, NB and other media with unspecified amounts of carbohydrates, proteins, nucleic acids and/or lipids. 26. An organism that is hard to cultivate. Has complex nutritional requirements. 29. Mixed-acid fermentation test in IMViC battery. 30. Autoclaving: minutes and psi 31. Proper alignment of the incident or illuminating light for microscopy. 33. One dye to elucidate shape, arrangement and sized. 38. Partial inhibition of GN growth. 39. Most critical step of the Gram stain procedure. 42. Hydrolytic enzyme produced by indole (+) organism. 43. Charged portion of the chromogen 45. Instrument used to observe colony characteristics. T, I, & T/I 46. Dry, moist, creamy or mucoid 48. Lab coat, gloves and goggles. 51. Crystal violet, bile salts, neutral red and lactose. 52. Non-reproductive biological process for protection/survival in unfavorable environmental conditions. 54. These salts inhibit GP organisms. 56. Present, appearing, or found everywhere 57. Poisonous, corrosive and combustible reagent used to detect indole. 58. Total magnification- using the oil immersion objective. 59. Medium used to determine susceptibility to dental caries.

Down 3. Non-inonic, gelatinous firmly attached glycocalyx. 5. Virulence of Gram-negative cells... true endotoxin. 6. Spread plate: for enumeration. 7. Phenol red, bromcresol green and bromthymol blue turn yellow at this pH. 8. Toxic by-product, of aerobic cellular respiration, degraded by catalase. 9. Hemolysis: clearing of medium: complete lysis of RBCs. 10. Club-shaped rods 14. Great Divider of Gram-Positive Cocci 15. Proper hand hygiene, in seconds. 17. Hemolysis: green medium: partial lysis of RBCs. 19. Enzyme produced by ALL obligate aerobes. 20. Principle component of bacterial cell wall. 24. Four-way streak or zigzag method. 25. Rapid hydrolyzer of urea: genus. 27. Tending to absorb moisture from air. 28. Thick pad-like growth on the surface of nutrient broth. 32. ________________ blue: indicator in SCA/ 34. Hydrophobic substance found in Acid-fast cell wall. 35. Information you collect with your senses (sight, touch, smell...) 36. Stain that imparts color to all cells. eg. crystal violet 37. Sulfanilic acid and alpha-Naphthylamine react with this acid to form p-Sulfobenzene-azo-alpha-naphthylamine. 40. Orientation, of agar plates, that prevent mixing of solid culture. 41. This lever changes the angle of illumination. 44. Free from contamination... technique. 47. Plate contains an acidic dye and a basic dye that partially inhibits the growth of GPs. 49. Deamination of this PRGB ingredient makes medium appear deep red or magenta. 50. Numerical aperture of the 10x objective 53. Agar concentration (%) of a solid nutrient gelatin broth/ gelatin deep. 55. Tube used to detect gas production, during carbohydrate fermentation.