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Across 8. Uses solar energy as their energy source 9. Prefer to grow in a pH of less than 7.0 13. Requires minute amounts of oxygen and die if there is too much 16. Cannot use oxygen to grow, grows by fermentation 17. There are _______ steps to the Gram stain 20. During this phase cell growth slows and the population stabilizes 22. Also called spontaneous generation 24. Anaerobic respiration may also be called ________ 27. A drug that inhibits or kills bacteria 29. The most deadly substance to man 30. Caused by the variola virus

Down 1. Most pathogens fit into this category of temperature requirements 2. ________ selects for gram negative bacteria and is a differential media 3. _______ viruses have no envelope 4. Aerobic respiration requires ________ 5. Caused by Yersinia pestis, also called "black death" 6. Three basic shapes of bacteria are bacilli, cocci, and _______ 7. ________ cells have a thick peptidoglycan layer 10. Most bacteria reproduce by ________ 11. _______ means it has carbon and hydrogen in it 12. Refers to increase in the size of colony, not cells 14. The primary dye used in acid-fast staining 15. To _______ while doing a Gram stain you use acetone/alcohol 18. Enzymes are made up of ________ 19. The bacterium has 1 or more flagella at each end of the cell 21. _______ staining uses an acidic dye to stain the background 23. Acid fast stains identify Mycobacterium and __________ 25. _______ media has extra vitamins, blood serum, or animal parts added 26. The most important factor to reduce contamination while making a pure culture 28. Gram negative cells stain this color