My Puzzles
Report bug
Collected Puzzles
User listed puzzles
Random Puzzle
Log In/Out

Molecular Mystery Puzzle

Dana McDaniel

1 2
3             4          
    5     6
7                           8
9     10        
11             12            
      14                 15
    16           17              
    18   19              

3.PCR is a method of target _______ which makes copies of target sequences so they may be detected
5.Differences in osmotic fragility, differences in density gradient, use of detergent, grinding, and homogenzing will _____ cells, releasing DNA.
7.It is critical for the DNA polymerase to be _________ to avoid denaturation at high temperatures
9.Reverse transcriptase PCR requires RNA to be first converted to _______
11.Southern and Northern blot methods are similar, in that they both use single-stranded __________ probes to identify sequences of interest
13.DNA and RNA can be eluted from a solid-phase matrix using _____
14.Probes must be labeled in order to _________ the target fragments immobilized on a blot
16.dsDNA is separated in PCR via __________ at 95 degrees Celsius
19.The accumilation of amplicons can be monitored during target amplification using _______ PCR.
20.Proteins may be analyzed using the _________ blot method
21.Another name for inorganic DNA extraction which selectively precipitates proteins.
23.DNA fragments may be isolated and detected using the __________ blot procedure
24.In conditions with high __________, the probe will only bind target sequences if they are complement to one another, while low conditions are more forgiving.
25.The ______________ is used to asses the progress of electrophoresis and aids in knowing when the run is complete (e.g. bromophenol blue).
26.Phenol and chloroform may be used in _______ DNA or RNA extraction
27.Extraction methods often involve ____ salt levels and ___ pH levels
1.In solid-phase extraction, DNA adsorbs to ____________
2.Anaylsis which requires detection and resolution of DNA down to the single-base level is best done using __________ gel
4.A ______ is used to create wells at the top of the gel in which samples will be loaded
6.DNA synthesis occurs in the _________ step of PCR
8.The _______ blot procedure may be used to anaylze the structure and quantity of RNA.
10.In contrast, Western blot uses _________ to identify sequences of interest
12.In the second step of PCR, primers/probes _____ to complementary sites on template DNA
15.Special care must be taken with RNA extraction due to high levels of _______ in the environment
16.Increasing the concentration of agarose in a gel will ________ the size of the spaces in the gel
17.__________ enzymes are used to cut DNA at four to six base pair binding sites
18.In PCR, DNA polymerase requires ______ for enzyme activity
22.During electrophoresis, buffers are used to carry the _______ in addition to protecting the samples

Use the "Printable HTML" button to get a clean page, in either HTML or PDF, that you can use your browser's print button to print. This page won't have buttons or ads, just your puzzle. The PDF format allows the web site to know how large a printer page is, and the fonts are scaled to fill the page. The PDF takes awhile to generate. Don't panic!

Web armoredpenguin.com

Copyright information Privacy information Contact us Blog